Scarlett Johansson Stars in New 'Captain America: Civil War' Trailer

Marvel Studios has released the latest trailer for the hugely anticipated Captain America: Civil War. The film is the latest entry in the mega-successful “Marvel Cinematic Universe,” which ties the various Marvel Studios films together in a shared continuity. Captain America: Civil War stars Chris Evans as Steve Rogers, aka Captain America, along with Amagansett’s Scarlett Johansson as Natasha Romanoff, aka the Black Widow. Civil War marks Johansson’s fifth time playing the assassin-turned-hero, and the latest trailer hints at the pivotal role she plays.

Captain America: Civil War pits the Avengers against each other after the government decides to regulate superpowered individuals following the catastrophic events of the other Marvel films (which include the devastating attack on New York at the hands of an alien army and the destruction of fictional European city Sokovia in the Avengers films). Rogers doesn’t believe in the government labeling people, while Tony Stark/Iron Man (played by Hamptons visitor Robert Downey, Jr.) thinks the new rules are necessary. The conflict escalates and the heroes come to blows. While Romanoff has been one of Captain America’s closest allies and best friends, it appears that she reluctantly stands by Iron Man in the trailer—though perhaps the former KGB spy is working as as Cap’s mole?

The trailer also shows Hampton fan Paul Rudd‘s character Ant-Man and offers the first look at the new Spider-Man, as played by Tom Holland. Marvel Studios recently partnered with Sony to bring the character in the Marvel Cinematic Universe following the lukewarm response to The Amazing Spider-Man 2, which starred Andrew Garfield as Spidey’s alter ego Peter Parker. Marvel is currently working on a new Spider-Man film with Holland, who is the third actor to play the web slinger after Tobey Maguire got the role in the first Spider-Man trilogy starting in 2002, and Garfield led the reboot with The Amazing Spider-Man in 2012. The MCU is once again starting from scratch with the Holland Spider-Man film.

Captain America: Civil War arrives in theaters on May 6, directed by Joe and Anthony Russo. Watch the trailer below.